Simultaneous analysis of the of levamisole with triclabendazole in pharmaceuticals through developing TLC and HPLC–PDA chromatographic techniques and their greenness assessment using GAPI and AGREE methods

Table 2 Parameters involved in evaluating the robustness of the developed HPLC method

Drug	Parameters		Retention time (t_R)	Capacity factor (K´)	Resolution (R)	Tailing factor (T)
Levamisole	Flow rate	1.1 mL/min	1.757	0.76	–-	1.19
		1 mL/min	1.771	0.77	–-	1.17
		0.9 mL/min	1.823	0.82	–-	1.19
	Mobile phase ratio (ACN: KH₂PO₄)	69:31	1.796	0.80	–-	1.19
		70:30	1.771	0.77	–-	1.17
		71:29	1.804	0.80	–-	1.19
Triclabendazole	Flow rate	1.1 mL/min	3.355	2.36	10.75	0.99
		1 mL/min	3.467	2.47	11.40	0.99
		0.9 mL/min	3.499	2.50	11.17	0.98
	Mobile phase ratio (ACN: KH₂PO₄)	69:31	3.505	2.50	11.42	0.97
		70:30	3.467	2.47	11.40	0.99
		71:29	3.314	2.31	10.27	0.98

ISSN: 2661-801X