Simultaneous analysis of the of levamisole with triclabendazole in pharmaceuticals through developing TLC and HPLC–PDA chromatographic techniques and their greenness assessment using GAPI and AGREE methods

Table 3 Parameters involved in evaluating the robustness of the developed TLC method

Drug	Parameters		Retention time (t_R)	Capacity factor (K´)	Resolution (R)	Tailing factor (T)
Levamisole	Detector wavelength	212 nm	0.72	0.395	–-	0.8
		215 nm	0.71	0.408	–-	0.8
		218 nm	0.71	0.408	–-	0.8
	Mobile phase ratio ethyl acetate: hexane: methanol: ammonia	68:15:16:1	0.71	0.395	–-	0.8
		69:15:15:1	0.71	0.408	–-	0.8
		70:15:14:1	0.71	0.408	–-	0.8
Triclabendazole	Detector wavelength	212 nm	0.76	0.301	1.29	0.9
		215 nm	0.77	0.298	1.33	1
		218 nm	0.78	0.296	1.31	0.9
	Mobile phase ratio ethyl acetate: hexane: methanol: ammonia	68:15:16:1	0.74	0.296	1.28	0.9
		69:15:15:1	0.77	0.298	1.33	1
		70:15:14:1	0.76	0.301	1.32	1

ISSN: 2661-801X