Table 1 Analytical performance data of the calibration graphs for the determination of FVM, and IVM by the proposed methods

Wavelength (nm)

270

272–258

∆A 270–290

240

254–291

∆A 245–260

Linearity range (µg/mL)

5–40

2.5–25

LOD (µg/mL)^a

0.7352

1.5306

0.4202

0.3995

0.0980

0.1213

LOQ (µg/mL)^b

2.2279

4.6381

1.2732

1.2106

0.2971

0.3677

Regression coefficient

0.99929

0.99966

0.99963

0.99947

0.99997

0.99995

Slope

0.0025

0.2521

0.0145

0.0008

0.0483

0.0350

SE of slope

0.0001

0.0114

0.0002

0.00001

0.0002

0.0002

Intercept

− 0.0011

0.2284

0.0130

0.0001

-0.0249

0.0005

SE of intercept

0.0006

0.1169

0.0019

0.0001

0.0014

0.0013