Stability indicating high performance thin layer chromatography method development and validation for quantitative determination of tetracycline hydrochloride in tetracycline hydrochloride active pharmaceutical ingredient (API) and its dosage forms

Table 2 Peak area of 400 ng/band tetracycline HCl in stress conditions for forced degradation study

Condition	Formulation	Concetration	Tº and time	Peak area	Percent^a comparision
STD@	Solution	0.1 mg/mL	Room Tº	0.00684	–
Oven 60 ºC	Powder	50 mg	60ºC for 6 h	0.00665	97.2
UV chamber	Powder	50 mg	254 nm light for 6 h	0.00627	91.7
0.1 M NaOH	Solution	0.1 mg/mL	80 ºC for 2 h	0.00373	54.5
0.1 M HCl	Solution	0.1 mg/mL	80 °C for 2 h	0	0
3.0% H₂O₂	Solution	0.1 mg/mL	Room Tº for 2 h	0	0

@: The standard (STD) at room temperature
^aPercent comparison is the peak area obtained in other conditions with that of the tandard room temperature condtion

ISSN: 2661-801X