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Table 3 Calibration parameters obtained using the HPTLC method

From: Stability indicating high performance thin layer chromatography method development and validation for quantitative determination of tetracycline hydrochloride in tetracycline hydrochloride active pharmaceutical ingredient (API) and its dosage forms

Parameter

HPTLC

Calibration range (ng/band

160–560

Coefficient of x2

− 7 × 10–9

Coefficient of x

2 × 10 −5

Y-intercept

− 0.0005

Determination coefficient (R2)

0.9999

Correlation coefficient (r)

0.99995

Polynomial regression equation

Y = − 7 × 10–8 x2 + 2 × 10–5 x-0.0005

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BMC Chemistry

ISSN: 2661-801X

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