BMC Chemistry

Table 7 Time gap between development and scanning effect on peak area of 320 ng (n = 4)

From: Stability indicating high performance thin layer chromatography method development and validation for quantitative determination of tetracycline hydrochloride in tetracycline hydrochloride active pharmaceutical ingredient (API) and its dosage forms

Time gap	Mean peak area	SD	%RSD	Reduction	Reduction (%)
5 min	0.00569	6.96E−05	1.223936	0	–
10 min	0.00566	3.09E−05	0.546149	− 3E−05	− 0.52724077
20 min	0.00564	4.11E−05	0.728654	− 5E−05	− 0.87873462
30 min	0.005485	4.15E−05	0.757213	− 0.00021	− 3.60281195
40 min	0.005408	7.05E−05	1.303548	− 0.00028	− 4.96485062
60 min	0.005443	7.05E−05	1.295165	− 0.00025	− 4.34973638
90 min^a	0.002323	4.49E−05	1.934575	− 0.00337	− 59.1827768

*Plate was exposed to light for 10 min

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ISSN: 2661-801X

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