Identification of isothiazolones analogues as potent bactericidal agents against antibiotic resistant CRE and MRSA strains

Table 1 MIC screening, cLogP and tPSA of isothiazol-3(2H)-ones and their combination with MRM at ratio of 1:1 against E. coli BL21 (NDM-1) strain and clinically isolated E. coli HN88 (NDM-1) strain

Entry	Compound				BL21		FICI	HN88		FICI	ClogP	TpsA
Entry	Compound				Cpd alone	MRM: Cpd	FICI	Cpd alone	MRM: Cpd	FICI	ClogP	TpsA
1	MRM	N.A	N.A	N.A	≥ 256	N.A	N.A	≥ 256	N.A	N.A	N.A	N.A
2	4a		H	H	2	1	0.503906	8	4	0.515625	2.472	20.31
3	4b		H	H	≥ 256	128	1	≥ 256	128	1	0.894	63.68
4	4c		H	H	≥ 256	≥ 256	2	≥ 256	128	1	2.171	20.31
5	5a		H	Cl	≤ 0.032	0.125	3.906738	2	2	1.007813	3.185	20.31
6	5b		H	Cl	≥ 256	128	1	≥ 256	128	1	1.607	63.68
7	5c		H	Cl	8	≤ 4	0.515625	32	16	0.5625	3.0238	29.54
8	5d		H	Cl	≥ 256	128	1	≥ 256	128	1	2.634	20.31
9	5e		H	Cl	≥ 256	64	0.5	≥ 256	128	1	1.575	66.81
10	5f	C₈H₁₇	H	Cl	4	≤ 1	0.253906	4	8	2.03125	− 4.203	20.31
11	5 g		H	Cl	0.064	0.125	1.9536	4	4	1.0156	0.703	20.31
12	6		Cl	Cl	≥ 256	32	0.25	≥ 256	≥ 256	2	3.778	20.31
13	7		Cl	H	8 or 16	2	0.257813	16	16	1.0625	3.185	20.31
14	8		Br	H	4	4	1.015625	32	16	0.5625	3.335	3.335
15	9		H	Cl	≥ 128	≥ 128	1.5	≥ 128	≥ 128	1.5	− 0.273	29.1

MIC value were determined using the double dilution method in accordance with the CLSI guidelines by which bactericide effect was assessed by naked eye after overnight incubation. All MIC experiments were performed at least triplicate

ISSN: 2661-801X