Skip to main content
Download PDF
Download ePub

Identification of isothiazolones analogues as potent bactericidal agents against antibiotic resistant CRE and MRSA strains

BMC Chemistry volume 17, Article number: 183 (2023) Cite this article

Abstract

Carbapenem-resistant Enterobacterales (CRE) has emerged as a worldwide spread nosocomial superbug exhibiting antimicrobial resistance (AMR) to all current antibiotics, leaving limited options for treating its infection. To discovery novel antibiotics against CRE, we designed and synthesized a series of 14 isothiazol-3(2H)-one analogues subjected to antibacterial activity evaluation against Escherichia coli (E. coli) BL21 (NDM-1) and clinical strain E. coli HN88 for investigating their structure–activity relationships (SAR). The results suggested that 5-chloroisothiazolone core with an N-(4-chlorophenyl) substitution 5a was the most potent antibacterial activity against the E. coli BL21 (NDM-1) with MIC value of less than 0.032 μg/mL, which was at least 8000-fold higher than the positive control Meropenem (MRM). It also displayed 2048-fold potent than the positive control MRM against E. coli HN88. Additionally, SAR analysis supported the conclusion that compounds with a chloro-group substituted on the 5-position of the heterocyclic ring was much more potent than other positions. The board spectrum analysis suggested that compound 5a showed a promising antimicrobial activity on MRSA and CRE pathogens. Meanwhile, cytotoxicity study of compound 5a suggested that it had a therapeutic index value of 875, suggesting future therapeutic potential. In vivo efficacy study declared that compound 5a could also protect the BALB/c mice against American type culture collection (ATCC) 43,300. Further screening of our compounds against a collection of CRE strains isolated from patients indicated that compound 5 g displayed much stronger antibacterial activity compared with MRM. In conclusion, our studies indicated that isothiazolones analogues could be potent bactericidal agents against CRE and MRSA pathogens.

Peer Review reports

Introduction

The serendipitous discovery of penicillin made by the Nobel laureate Alexander Fleming inaugurated the modern era of antibiotic utilization [1]. Since then lots of beta-lactam antibiotics, containing a beta-lactam ring as the scaffold, have been obtained. The beta-lactam antibiotics such as penicillins, cephalosporins, carbapenems and monobactams have been a cornerstone to treat infections caused by Gram-negative bacterial pathogen due to their efficacy and low toxicity to humans [2]. To date, more than 50% percent of antibiotics in clinical use belonged to β-lactam [3, 4]. Unfortunately, the Center for Disease Control (CDC) declared that 50% of antibiotics prescriptions in the hospital setting and nursing homes were either inappropriate or unnecessary [5, 6]. Eventually, the abuse of the antibiotics eventually led to the emergence of bacterial resistance. The expression of beta-lactamases rendering resistance to beta-lactam antibiotics by breaking the beta-lactam ring that is essential for the bactericidal activity posed a serious threat to human health (Fig. 1) [7].

Fig. 1
figure 1

Antimicrobial resistance: β-lactamase modification (ESKAPE referred to Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa and Enterobacter spp.)

Full size image

Based on the different amino acid sequences and functional mechanisms, beta-lactamases can be classified into four classes: Three serine-dependent enzyme classes A, C, and D known as serine-β-lactamases (SBLs) employed an active site serine to nucleophilically attack on the β-lactam carbonyl [8]. Widely accepted inhibitors of SBLs have been used in clinical such as sulbactam, tazobactam, and clavulanic acid [9]. One metal-dependent enzyme class B called metallo-β-lactamases (MBLs) adopted zinc-bound hydroxyl to nucleophilically attack the carbonyl group of β-lactam (Fig. 2) [10]. Clinically proven inhibitors of MBLs are still unavailable up to date [11]. Still worse, MBLs are not only encoded by horizontally transferable plasmids but also associated commonly with genes encoding for other antibiotic resistance determinants, conferring Carbapenem-resistant Enterobacterales (CRE) ‘‘superbugs’’ which exhibits antimicrobial resistance (AMR) to nearly all current antibiotics [12]. What’s more, the CDC has manifested that the golden age of carbapenem set to end due to the “ESKAPE superbugs” which confer infections that require development of new effective antibiotics for treatment [13]. In particular, the rapid worldwide dissemination of NDM-1-producing“superbugs” further emphasizes the significant role of this type of carbapenemases in conferring antimicrobial resistance.

Fig. 2
figure 2

Classification and Mechanism of beta-lactamases

Full size image

Recently the tremendous effort to discover potential NDM-1 inhibitors have been made [14]. Numerous NDM-1 inhibitors have been reported, but none of them have been approved by FDA [15]. Only cyclic boronate derivatives including VNRX-5133 and QPX7728 entered phase III and phase I clinical trial, respectively [16, 17]. Besides the approach to discover NDM-1 inhibitors to treat the currently untreatable infections by CRE, an alternative reliable strategy is to find a novel antibiotic with the ability to fight antibiotic resistance [18]. During the period of searching for the NDM-1 inhibitors, we have also disclosed a novel series of potent antibiotics referred to isothiazolones analogues [7, 19, 20]. The isothiazolone analogues reported before have displayed broad-spectrum antibacterial activity against Gram-negative and Gram-positive strains [21, 22]. In this paper, we further expanded the scope of compounds and determined whether their bactericidal effects against non-resistance pathogens could also be replicated in a panel of CRE and MRSA and investigated structure-antibacterial activity relationships of isothiazolones against inducible Carbapenem-resistant E. coli BL21 carrying Pet28-blaNDM-1 and clinical isolated strain E. coli HN88 carrying blaNDM-1.

Results and discussion

Chemistry

The synthetic route for the preparation of a collection of N-functionalized isothiazolones derivatives reported previously was outlined in Fig. 3 [23]. Starting from commercially available 3,3'-disulfanediyldipropionic acid 1, treatment with thionyl chloride in Dichloromethane (DCM) solvent with N,N-Dimethylformamide (DMF) as catalyst afforded the acyl chloride 2, which was further reacted with excess corresponding amine gave the dithiodipropionamides 3, followed by subsequent chlorine-assisted cyclization of the diamide with sulfuryl chloride using DCM as a solvent in an ice bath afforded the desired isothiazol-3(2H)-ones 4–6 [24]. Upon modification of the reagent stoichiometry, various products could be obtained as it was suggesting that the 5-unsubstituted analogues 4 were the single products with moderate yields when the ratio of diamide to sulfuryl chloride was 1:1, the 5-chloroisothiazolone derivatives 5 were the predominant products with 4-chloroisothiazolone derivatives 7 as the side products while with the relevant ratio of 1:3, and while with the ratio of 1:5 afforded the 4,5-dichloroisothiazolone derivatives 6 as the major product. Further bromination of compound 4a gave 4-bromoisothiazolone derivative 8. Oxidation of 5-chloroisothiazolone compound 5a with 3-chloroperoxybenzoic acid (m-CPBA) in an ice bath gave the 5-chloroisothiazol-3(2H)-one-1-oxide 9 in a low yield.

Fig. 3
figure 3

Synthesis route of designed compounds. Regents and conditions: a SOCl2, DMF, DCM, reflux, 1 h; b corresponding amine, Et3N, DCM, ice bath to r. t, 18 h; c SO2Cl2, DCM, ice bath to r. t, 18 h; d Br2, EA, ice bath to r. t, overnight; e mCPBA, CH2Cl2, r.t, overnight

Full size image

In vitro bactericidal screening

Cell-based bactericidal screen using E. coli BL21 (NDM-1) and clinical strain E. coli HN88 identified compound 5a as a promising antibiotic against superbugs.

MRM-resistant E. coli BL21 (NDM-1) carrying only an Isopropyl-β-D-thiogalactopyranoside (IPTG)-inducible plasmid pET28b-bla NDM-1 was produced from a parental E. coli BL21 strain without producing NDM-1. E. coli HN88 carrying blaNDM-1 was collected from clinical patients. All cell-based bactericidal screen using E. coli BL21 (NDM-1) and clinical strain E. coli HN88 were conducted according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. The minimum inhibition concentration (MIC) of MRM towards these antibiotic resistance strains of E. coli BL21 (NDM-1) and E. coli HN88 were found to be greater than 256 μg/mL (Table 1, entry 1), which was at least 2056-fold higher than the parental E. coli BL21 with antibiotic susceptibility (MIC of MRM = 0.125 μg/mL).

Table 1 MIC screening, cLogP and tPSA of isothiazol-3(2H)-ones and their combination with MRM at ratio of 1:1 against E. coli BL21 (NDM-1) strain and clinically isolated E. coli HN88 (NDM-1) strain
figure a
Full size table

Structure–activity relationship study of isothiazol-3(2H)-ones for bactericidal

A total of 14 isothiazol-3(2H)-one analogues were designed, synthesized and simultaneously subjected to assessment of bacterial-killing activity against E. coli BL21 (NDM-1) and clinical strain E. coli HN88 for investigating their structure–activity relationship. In this study, structural modification of these novel isothiazol-3(2H)-one analogues were mainly focused on its 4- and 5- substituents positions, as well as the N-position (Fig. 3). These antibacterial screen results were summarized as shown in Table 1. MRM was selected to act as positive control (Entry 1, Table 1).

In order to reveal the functional scaffold for the antibacterial activity of isothiazol-3(2H)-ones, several series isothiazolones with N-position groups different in hydrophobicity, group size, steric and electronic parameters were synthesized and evaluated of their antibiotic activity against the resistant strains. Most compounds showed ignored or no antibacterial effect with MIC ≥ 8 μg/mL except compound 4a, 5a, 5f, 5g and 8, indicating that compounds with N-phenyl ring substituted with 4-chloro group would be more beneficial such as compound 4a and 5a. Next, by analysis of the MIC values of compound 4a, 5a, 6, 7 and 8 with the same N-phenyl ring substituted with 4-chloro group, we could draw the conclusion that 5-chloroisothiazolone core with an N-(4-chlorophenyl) substitution 5a had the highest antibacterial activity among the corresponding C-5 unsubstituted analogues (compound 4a, 7 and 8) and C-4,5 dichloro-substituted analogue 6. Furthermore, compound 5a (Entry 7, Table 1) showed the most potent antibacterial activity against the E. coli BL21 (NDM-1) with MIC value of less than 0.032 μg/mL, which was at least 8000-fold higher than the positive control MRM. It was also referred to E. coli HN88, suggesting that 2048-fold higher than the positive control MRM. Nevertheless, compound 9 obtained by oxidation of 5a (Entry 18, Table 1) showed MIC value ≥ 128 μg/mL, suggesting that 5-chloroisothiazolone core was indispensable pharmacophore for bactericidal effect. Besides, for the E. coli BL21 carrying blaNDM-1, the MIC value of compound 5a was found to be ≤ 0.032 μg/mL, conferring a more than 62.5-fold reduction in E. coli HN88 MIC which was 2 μg/mL, suggesting that the clinical strain was more resistant than the experimental one. Therefore, we selected compound 5a as the lead candidate for further investigation on bactericidal effects against a panel of clinical isolated strains carrying various MBLs.

Synergistic study of tested compounds with conventional antibiotic MRM.

The combinational usage of two bactericidal agents could boost the bacterial susceptibility and prevent the antimicrobial resistance [25, 26]. Therefore, we next determined whether the isothiazol-3(2H)-ones had synergistic effect with conventional antibiotic MRM by incubating the E. coli BL21 (NDM-1) strain and clinically isolated E. coli HN88 (NDM-1) strain in the presence of tested compounds and MRM at the ratio of 1:1. The synergistic effect was determined by the FIC index, which was calculated as FIC (cpd) + FIC (MRM), where FIC (cpd) was defined as the (MICcombination)/(MICcpd) and FIC (MRM) was the ratio of MICcombination to MICMRM. The drug combination was considered synergy if FIC Index was ≤ 0.5, no interaction if 0.5 < FICI ≤ 4, and antagonism if FICI > 4. The results as shown in Table 1 suggested that nearly all of the synthetic isothiazol-3(2H)-ones derivatives had no synergistic effect with MRM against the aforementioned strains with exception of compound 5f, 6 and 7 with FICI value of approximately 0.25 on the E. coli BL21 (NDM-1) strain. The FICI between compound 5a and MRM were larger than 3.9 on the E. coli BL21 (NDM-1) strain, indicating this compound 5a had antagonistic effect with MRM.

Spectrum of activity of compounds 4a, 5a and 5 g

In order to demonstrate the board spectrum of the most potent compounds 4a, 5a and 5 g, several ATCC strains including MRSA were used in the antimicrobial susceptibility testing (Table 2). The results suggested that compound 5a showed a promising antimicrobial activity on Gram-negative pathogen E. coli and Gram-positive pathogen S. aureus. Compound 5 g could kill the Gram-positive pathogen S. aureus with MIC value of 2 µg/mL.

Table 2 MIC (µg/mL) screening of compounds 4a, 5a and 5g against on ATCC strains
Full size table

MIC screening of compound 5a against clinically isolated Gram-negative CRE strains

On the basis of the MIC screening results of isothiazol-3(2H)-ones against E. coli BL21 (NDM-1) strain and E. coli HN88 (NDM-1) strain, we further checked whether their bactericidal effects in the aforementioned screening strains could also be reproduced in our in-house collection of a panel of 5 Gram-negative CRE strains including E. coli, C. freundii, E. cloacae, K. pneumoniae and M. morganii strains which were clinically isolated from patients in the Second People’s Hospital of Jiaxing in Zhejiang Province. These CRE strains are all NDM-1 positive and highly resistant to MRM, exhibiting MICs of MRM ranging from 64 µg/mL to ≥ 128 µg/mL (Table 2). Apart from expressing NDM-1 enzyme, all of the tested strains with exception of M. morganii could also produce other additional β-lactamases such as CTX-M-3, CTX-M-14, SHV-12, TEM-1 and KPC-2 to confer antimicrobial resistance (AMR). Encouragingly, as illustrated in Table 3, four compound including 4a, 5f, 5 g and 7 demonstrated promising antibacterial activity itself (MICs ≤ 32 µg/mL), exhibiting much stronger antibacterial activity against the aforementioned pathogens compared with the positive compound MRM and else compounds.

Table 3 MIC (µg/mL) screening of MRM and isothiazol-3(2H)-ones against clinically isolated CRE strains carrying NDM-1 and additional β-lactamases
Full size table

Growth curve and time-killing assay of compound 5a against S. aureus 43,300

Time-killing assay used to unveil the dynamic interaction between antimicrobial agents and strains is the most useful method for determining the bactericidal effects, revealing a time-dependent and a concentration-dependent bactericidal effects [27, 28]. To determine the bactericidal effect of compound 5a, the growth curve assay and the time-killing assay against S. aureus 43,300 were conducted against Gram-positive strain S. aureus 43,300. The growth curve of the treatment of compound 5a at MIC concentration displayed a gradual log reduction with OD600 value ranging from 0.159 to 0.001 within 4 h. Furthermore, incubating of S. aureus with compound 5a at > twofold MIC suggested that no colonies formed at 4, 6, 8 and 22 h (Fig. 4A, B). In addition, the bacterial survival rates of S. aureus 43,300 exposed to compound 5a at various concentrations ranging from 1/4 MIC to 4 MIC at different times (Fig. 4C) were measured. The aftermath suggested that any concentration of 5a could not kill bacteria within 4 h, and only fourfold MIC of 5a could kill most bacteria within 22 h.

Fig. 4
figure 4

Anti-MRSA activity of compound 5a in vitro. A Image of incubation of S. aureus with compound 5a (B) Growth curve of compound 5a against S. aureus 43,300. The X-axis shows the time (h), and the Y-axis represents the OD600. C Time-killing assay of compound 5a against S. aureus 43,300. Data are mean ± SEM for three independent experiments

Full size image

Cytotoxicity studies of compounds against eukaryotic cells

Given that the cytotoxicity towards eukaryotic cells has been the greatest obstacle to the development of bactericidal, mouse peritoneal fibroblast L929 cell line was selected to verify the safety of the most potent compound 5a. As shown in Fig. 5A, compound 5a exhibited relatively low toxicity against L929 cell lines with IC50 value of 3.5 ± 0.7 μM, which is much higher than the MIC of 0.004 μM (1.0 μg/mL). Hence the therapeutic index calculated by IC50/MIC was 875, indicating that compound 5a has a very broad therapeutic window. Moreover, cell morphology microscopic analysis suggested that no obvious morphological changes of L929 cells were observed after prolonged incubation with compound 5a at the MIC, exhibiting negligible toxicity. In addition, compound 4a also displayed relatively low toxicity against L929 cell lines with IC50 value of 8.7 ± 1.0 μM as shown in Fig. 5B, suggesting that this series of compounds could have sufficient margins of safety.

Fig. 5
figure 5

Cytotoxicity of compound 5a (A) and 4a (B) against L929 cell lines

Full size image

In vivo bactericidal action study of compound 5a

To shed light on the potential clinical benefits of compound 5a, in vivo efficacy was determined using a BALB/c mice infection model. The therapeutic abilities of compound 5a at single doses of 0.5 mg/kg in drug monotherapy to protect the mice against a lethal dose infection of MRSA ATCC43300 (109 CFU/mouse) through intravenous injection was evaluated. 70% mortality of mouse were observed for the control group of the vehicle consisting of 5% Cremophor EL, 5% ethanol and 90% saline after 24 h. Encouragingly, 0.5 mg/kg monotherapy of compound 5a after 24 h resulted in 80% survival rate, suggesting that the excellent antibacterial ability of compound 5a against MRSA ATCC43300 (Fig. 6). Compared to the treatment groups of the vehicle control group, it was found to be highly significant (p < 0.05). These results suggested that compound 5a with strong potential should be worthy for further development in future.

Fig. 6
figure 6

Kaplan–Meier survival curves of MRSA infected mouse following the injection of vehicle solution and compound 5a. Data are the means of three independent experiments

Full size image

Conclusion

In this study, a total of 14 compounds isothiazol-3(2H)-one analogues were designed, synthesized and simultaneously subjected to assessment of bacterial-killing activity against E. coli BL21 (NDM-1) and clinical strain E. coli HN88. The SAR study suggested that 5-substituted chloride of the isothiazol-3(2H)-one enjoyed the priority. Compound 5a displayed the most potent in vitro antibacterial activity against MRSA and CRE with the broad spectrum. In vivo study also suggested compound 5a could enhance the survival rate in BALB/c mice infection model. Compound 5g even could kill several clinical isolated CRE strains carrying various MBLs which was clinically isolated from patients. Altogether, our studies indicate that isothiazol-3(2H)-one derivatives provided a promising starting point to be further developed as broad-spectrum antibiotics against superbugs.

Experimental section

General

Starting materials and reagents of commercial grade could be directly employed without further purification unless otherwise stated. All common reactions were visualized by TLC on aluminum sheets (Silica gel 60-F254, E. Merck) under UV light at 254 nm. Flash chromatography was performed on silica-gel 60 (200–300 mesh). 1H NMR (400 MHz) and 13C NMR (100 MHz) spectra were measured on a Bruker Advance-III spectrometer with TMS as an internal standard. Chemical shifts are expressed in δ (ppm) and coupling constants (J) in Hz (Additional file 1). High resolution MS spectra were measured using a QTOF-2 micromass Spectrometer by electron spray ionization.

To a well-stirred DCM solution (30 mL) of commercially available 3,3′-disulfanediyldipropanoic acid 1 (2.0 g, 9.52 mmol) was added dropwise thionyl chloride (2.48 g, 20.92 mmol) following dropping into DMF as the catalyst at 0 ◦C. The solution was heated under reflux for 12 h, the solvent was removed in vacuo and the crude residue was further reacted with corresponding amine (11.04 mmol) in DCM. After 6 h, the precipitate was filtered, washed with DCM, and dried in vacuo to yield the targeted diamide compound with sufficient purity for the next step. To a well-stirred solution of the diamide compound in DCM (25 mL) was added 1.0 equiv. of SO2Cl2 in an ice bath. After 2 h, the solution was poured into water and extracted with DCM. The organic layers were dried over anhydrous MgSO4, filtered and evaporated under reduced pressure. The resulting residue was purified by flash column chromatography on silica gel to afford the desired compound 4a–4c as the major product.

2-(4-chlorophenyl)isothiazol-3(2H)-one (4a)

This compound (yield 47.6%) was prepared from the 3,3'-disulfanediylbis(N-(4-chlorophenyl)propanamide) 3a (0.43 g, 1.0 mmol) and sulfuryl chloride (0.13 g, 1.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 8.15–8.25 (m, 1H), 7.48–7.56 (m, 2H), 7.35–7.42 (m, 2H), 6.29 (d, J = 6.85 Hz, 1H); 13C NMR (101 MHz, CHLOROFORM-d) δ 165.3, 146.6, 134.3, 133.4, 129.7, 125.9, 114.9. HRMS m/z calcd for [C9H6ClNOS + H] + 211.9931, found 211.9926.

2-((4-methoxyphenyl)sulfonyl)isothiazol-3(2H)-one (4b)

This compound (yield 64.5%) was prepared from the 3,3'-disulfanediylbis(N-((4-methoxyphenyl)sulfonyl)propanamide) 3b (0.55 g, 1.0 mmol) and sulfuryl chloride (0.13 g, 1.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 8.59 (d, J = 4.89 Hz, 1H), 7.76–7.96 (m, 2H), 6.93–6.99 (m, J = 8.80 Hz, 2H), 6.88 (d, J = 4.89 Hz, 1H), 3.82 (s, 3H); 13C NMR (101 MHz, CHLOROFORM-d) δ 164.5, 159.6, 151.4, 130.9, 126.5, 115.2, 114.5, 55.8. HRMS m/z calcd for [C9H6ClNOS + Na] + 293.9865, found 293.9860.

2-(4-bromobenzyl)isothiazol-3(2H)-one (4c)

This compound (yield 39.1%) was prepared from the 3,3'-disulfanediylbis(N-(4-bromobenzyl)propanamide) 3c (0.55 g, 1.0 mmol) and sulfuryl chloride (0.13 g, 1.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 8.42 (d, J = 3.91 Hz, 1H), 7.44—7.65 (m, J = 8.80 Hz, 2H), 7.21—7.44 (m, J = 8.80 Hz, 2H), 6.65 (d, J = 4.89 Hz, 1H), 5.40 (s, 2H); 13C NMR (101 MHz, CHLOROFORM-d) δ 169.2, 149.1, 135.7, 131.7, 129.8, 122.2, 111.9, 77.6, 77.3, 77.0, 69.7, 31.7, 22.8, 14.3. HRMS m/z calcd for [C10H8BrNOS + H] + 269.9510, found 269.9524.

To a well-stirred solution of the diamide compound in DCM (25 mL) and 4.5 equiv. Et3N was added 3.0 equiv. of SO2Cl2 in an ice bath. After 2 h, the solution was poured into water and extracted with DCM. The organic layers were dried over anhydrous MgSO4, filtered and evaporated under reduced pressure. The resulting residue was purified by flash column chromatography on silica gel to afford the desired compound 5a–5h as the major product and 7 as the side compound.

5-chloro-2-(4-chlorophenyl)isothiazol-3(2H)-one (5a)

This compound (yield 60.5%) was prepared from the 3,3'-disulfanediylbis(N-(4-chlorophenyl)propanamide) 3a (0.43 g, 1.0 mmol) and sulfuryl chloride (0.39 g, 3.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 7.45–7.54 (m, 2H), 7.37–7.45 (m, 2H), 6.37 (s, 1H); 13C NMR (101 MHz, CHLOROFORM-d) δ 167.5, 140.2, 135.1, 133.0, 129.5, 125.9, 114.7. HRMS m/z calcd for [C9H5Cl2NOS + H] + 245.9469, found 245.9492.

4-chloro-2-(4-chlorophenyl)isothiazol-3(2H)-one (7)

This compound (yield 28.6%) as a side product was prepared from the 3,3'-disulfanediylbis(N-(4-chlorophenyl)propanamide) 3a (0.43 g, 1.0 mmol) and sulfuryl chloride (0.39 g, 3.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 8.14 (s, 1H), 7.53–7.60 (m, J = 8.80 Hz, 2H), 7.41–7.48 (m, 2H); 13C NMR (101 MHz, CHLOROFORM-d) δ 162.2, 135.2, 133.6, 132.6, 129.7, 129.6, 125.5, 115.2. HRMS m/z calcd for [C9H5Cl2NOS + H]+ 245.9542, found 245.9540.

5-chloro-2-((4-methoxyphenyl)sulfonyl)isothiazol-3(2H)-one (5b)

This compound (yield 64.2%) was prepared from the 3,3'-disulfanediylbis(N-((4-methoxyphenyl)sulfonyl)propanamide) 3b (0.55 g, 1.0 mmol) and sulfuryl chloride (0.39 g, 3.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 7.87 (d, J = 9.78 Hz, 2H), 7.00 (d, J = 8.80 Hz, 2H), 6.87 (s, 1H), 3.86 (s, 3H); 13C NMR (101 MHz, CHLOROFORM-d) δ 164.7, 157.9, 155.1, 131.0, 126.3, 115.4, 114.6, 77.6, 77.3, 76.9, 55.8. HRMS m/z calcd for [C10H8ClNO4S2 + Na] + 327.9475, found 327.9463.

5-chloro-2-(3-chloro-4-methoxyphenyl)isothiazol-3(2H)-one (5c)

This compound (yield 57.5%) was prepared from the 3,3'-disulfanediylbis(N-(3-chloro-4-methoxybenzyl)propanamide) 3f (0.52 g, 1.0 mmol) and sulfuryl chloride (0.39 g, 3.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 7.44 (d, J = 2.93 Hz, 1H), 7.29 (dd, J = 2.45, 8.31 Hz, 1H), 7.02 (d, J = 8.80 Hz, 1H), 6.83 (s, 1H), 3.95 (s, 3H); 13C NMR (101 MHz, CHLOROFORM-d) δ 163.7, 157.4, 156.0, 129.9, 127.7, 124.9, 124.3, 123.6, 112.5, 77.4, 77.1, 76.8, 56.5. HRMS m/z calcd for [C10H7Cl2NO2S + Na] + 297.9467, found 297.9474.

5-chloro-2-cyclohexylisothiazol-3(2H)-one (5d)

This compound (yield 74.5%) was prepared from the 3,3'-disulfanediylbis(N-cyclohexylpropanamide) 3g (0.37 g, 1.0 mmol) and sulfuryl chloride (0.39 g, 3.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 6.13 (s, 1H), 4.16–4.40 (m, 1H), 1.84–2.13 (m, 3H), 1.75 (d, J = 5.87 Hz, 2H), 1.60 (d, J = 13.69 Hz, 1H), 1.18–1.48 (m, 4H); 13C NMR (101 MHz, CHLOROFORM-d) δ 166.3, 145.5, 115.0, 77.6, 77.3, 76.9, 53.3, 33.1, 25.4, 25.0. HRMS m/z calcd for [C9H12ClNOS + H] + 218.0328, found 211.0395.

5-chloro-2-(quinolin-8-ylsulfonyl)isothiazol-3(2H)-one (5e)

This compound (yield 40.6%) was prepared from the 3,3'-disulfanediylbis(N-(quinolin-8-ylsulfonyl)propanamide) 3h (0.59 g, 1.0 mmol) and sulfuryl chloride (0.39 g, 3.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 9.05–9.16 (m, 1H), 8.56 (d, J = 6.85 Hz, 1H), 8.29 (d, J = 7.82 Hz, 1H), 8.19 (d, J = 7.82 Hz, 1H), 7.68 (t, J = 7.83 Hz, 1H), 7.52–7.63 (m, 1H), 7.04 (s, 1H); 13C NMR (101 MHz, CHLOROFORM-d) δ 158.0, 154.7, 152.2, 143.8, 136.7, 135.7, 133.7, 129.0, 125.3, 122.7, 115.8. HRMS m/z calcd for [C12H7ClN2O3S2 + H] +326.9587, found 326.9512.

5-chloro-2-octylisothiazol-3(2H)-one (5f)

This compound (yield 42.7%) was prepared from the 3,3'-disulfanediylbis(N-octylpropanamide) 3i (0.43 g, 1.0 mmol) and sulfuryl chloride (0.39 g, 3.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 6.20 (s, 1H), 3.67 (t, J = 7.34 Hz, 2H), 1.53–1.78 (m, 2H), 1.17—1.32 (m, 10H), 0.72–0.95 (m, 3H); 13C NMR (101 MHz, CHLOROFORM-d) δ 166.8, 145.4, 114.7, 43.7, 31.7, 29.6, 29.0, 29.0, 26.4, 22.6, 14.0. HRMS m/z calcd for [C11H18ClNOS + H] + 248.0870, found 248.0872.

5-chloro-2-(prop-2-yn-1-yl)isothiazol-3(2H)-one (5 g)

This compound (yield 46.1%) was prepared from the 3,3'-disulfanediylbis(N-octylpropanamide) 3h (0.43 g, 1.0 mmol) and sulfuryl chloride (0.39 g, 3.0 mmol) according to the general procedure described above. 1H NMR (400 MHz, CHLOROFORM-d) δ 6.25 (s, 1H), 4.51 (d, J = 1.96 Hz, 3H), 2.49 (t, J = 2.45 Hz, 1H); 13C NMR (101 MHz, CHLOROFORM-d) δ 166.3, 147.2, 114.2, 76.6, 75.3, 33.0. HRMS m/z calcd for [C6H4ClNOS + H] +173.9702, found 173.9756.

4,5-dichloro-2-(4-chlorophenyl)isothiazol-3(2H)-one (6)

To a well-stirred solution of the 3,3'-disulfanediylbis(N-(4-chlorophenyl)propanamide) 3a (0.43 g, 1.0 mmol) in DCM (25 mL) was added 5.0 equiv. of SO2Cl2 (0.65 g, 5.0 mmol) in an ice bath. After 2 h, the solution was poured into water and extracted with DCM. The organic layers were dried over anhydrous MgSO4, filtered and evaporated under reduced pressure. The resulting residue was purified by flash column chromatography on silica gel to afford the desired compound 6a. 1H NMR (400 MHz, CHLOROFORM-d) δ 7.48–7.58 (m, 3H), 7.38–7.48 (m, 2H); 13C NMR (101 MHz, CHLOROFORM-d) δ 160.4, 139.3, 134.4, 133.9, 129.9, 125.7, 115.3. HRMS m/z calcd for [C9H4Cl3NOS + H]+ 279.9152, found 279.9151.

4-bromo-2-(4-chlorophenyl)isothiazol-3(2H)-one (8)

To a well-stirred solution of the 2-(4-chlorophenyl)isothiazol-3(2H)-one 4a (0.21 g, 1.0 mmol) in DCM (25 mL) was added 1.0 equiv. of Br2 (0.13 g, 1.0 mmol) in an ice bath. After 2 h, the solution was poured into water and extracted with DCM. The organic layers were dried over anhydrous MgSO4, filtered and evaporated under reduced pressure. The resulting residue was purified by flash column chromatography on silica gel to afford the desired compound 8. 1H NMR (400 MHz, CHLOROFORM-d) δ 8.25 (s, 1H), 7.56 (d, J = 7.82 Hz, 2H), 7.40–7.51 (m, 2H); 13C NMR (101 MHz, CHLOROFORM-d) δ 162.9, 135.3, 135.2, 133.6, 129.7, 125.5, 102.6. HRMS m/z calcd for [C9H5BrClNOS + H] + 289.9037, found 289.9047.

5-chloro-2-(4-chlorophenyl)isothiazol-3(2H)-one 1-oxide (9)

To a well-stirred solution of the 5-chloro-2-(4-chlorophenyl)isothiazol-3(2H)-one 5a (0.25 g, 1.0 mmol) in DCM (25 mL) was added dropwise 3-chloroperoxybenzoic acid (0.21 g, 1.2 mmol) in DCM in an ice bath. After 3 h, the solution was poured into water and extracted with DCM. The organic layers were dried over anhydrous MgSO4, filtered and evaporated under reduced pressure. The resulting residue was purified by flash column chromatography on silica gel to afford the desired compound 9. 1H NMR (400 MHz, CHLOROFORM-d) δ 7.44–7.51 (m, 2H), 7.34–7.41 (m, 2H), 6.85 (s, 1H); 13C NMR (101 MHz, CHLOROFORM-d) δ 163.5, 157.3, 135.3, 131.1, 130.1, 128.7, 124.3, 77.4, 77.1, 76.8. HRMS m/z calcd for [C9H5Cl2NO2S + H] +261.9418, found 261.9408.

Antimicrobial susceptibility testing

MRM-resistant E. coli BL21 (NDM-1) carrying only an IPTG-inducible plasmid pET28b-bla NDM-1 was produced from a parental E. coli BL21 strain without producing NDM-1 for MIC determination of tested compounds. E. coli HN88 carrying blaNDM-1 was isolated from urine specimens of urinary tract infected patient. CRE strains shown in Table 3 were collected from the patients’ urine, feces, and sputum in the Second People’s Hospital of Jiaxing in Zhejiang Province, China. It should be noted that humans were not involved in the current study. The aforementioned isolated strains were provided by the hospital. Strains including E. coli and S. aureus in Table 2 were purchased from the American Type Culture Collection (ATCC, Manassas, VA).

MIC determination

A collection of E. coli BL21 (NDM-1) were incubated overnight on the Mueller–Hinton agar (MHA) plate at 37 °C under aerobic conditions followed by transferring to normal saline (NS) where the OD600 value of the NS solution ranged from 0.08 to 0.1. The NS solution consisting of E. coli BL21 (NDM-1) was then transferred again to a 96-well plate and incubated with Mueller–Hinton broth (MHB), 1 mM of IPTG, and a serial concentration of MRM alone, the freshly prepared compound alone in DMSO, or a combination of both at the ratio 1 to 1. After being incubated at 37 °C overnight, the MIC values of the 16 antimicrobial agents were determined using a broth microdilution method following CLSI guidelines and our previous study. [7, 19, 20]

Cytotoxicity (IC50) test of compound 5a towards the L929 Cell Line

According to the method reported before, the standard 3-(4,5-dimethylthiazol-2-yl)-5(3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay was performed to evaluate the cytotoxicity of most potent compound 5a against the mouse peritoneal fibroblast L929 cells [19, 20, 29]. The half-maximal inhibitory concentration (IC50) of compound 5a was measured by using a Cell Titer 96 AQueous assay. Briefly, L929 Cells at a density of 10,000 cells were exposed to various concentrations compound 5a in a final volume of 100 μL in each well of a 96-well plate, followed by 48 h incubation at 37 °C. The negative group was comprised of a cell mixture containing 0.1% DMSO and the blank group consisted of the DMEM (10% FBS) medium alone without cells. The medium was removed from the plates after treatment with compound 5a, followed by addition of the freshly prepared MTS/phenazine methosulfate mixture at a ratio of 20:1 in PBS and DMEM into each well and further incubation for 2 h at 37 °C. The optical density was determined at 490 nm by a Microplate Reader (Clariostar, BMG). Percentage cell survival was calculated as follows: (corrected reading from the test well–corrected reading from the blank well)/ (corrected reading from negative well–corrected reading from the blank well) × 100%. The IC50 value of compound 5a was estimated from the dose−response curve of the MTS assay by using software GraphPad Prism 9. All experiments were performed in triplicates.

Growth curve of compound 5a against S. aureus 43,300

An inoculum size of Gram-positive strain S. aureus 43,300 was incubated at 37 ºC in the presence of compound 5a at the concentrations equal to 1/4 MIC, 1/2 MIC, MIC, twice the MIC, and four times the MIC for overnight. A control test was performed for the organisms without compound 5a. Aliquots of 1.0 mL of the samples were collected at time intervals of 0, 4, 6, 8, 12, and 22 h and inoculated aseptically with subjection to a series of tenfold dilution on brain–heart infusion (BHI) broth plates followed by incubating at 37 ºC for 24 h. Their growth was checked by measuring the absorbance at 600 nm. The procedure was performed in triplicate and a graph of the CD600 against time was plotted.

Time-dependent killing assay

The bacterial suspensions of MRSA 43300 were adjusted in Luria–Bertani broth (LB) to 106 CFU/mL, followed by treatment with the above-mentioned concentrations of compound 5a at 37 °C with continuous shaking (200 rpm). Viable bacterial cells at each time point (0, 4, 8, 12, and 22 h) were counted. The procedure was performed in triplicate and the time-killing curve were plotted using GraphPad 8.0 (San Diego, CA, USA).

Evaluation of the in vivo antibiotic activity using a BALB/c mice model of MRSA infection

To evaluate the in vivo bactericidal efficacy of compound 5a, a MRSA infection model of BALB/c mice was employed as previously described with little modification [7, 30,31,32]. MRSA infection mouse was a successful whole-animal model for screening antibacterial activities of compounds. The animal study was performed in full compliance with the standard protocol approved by the animal research ethics committee of Yunnan University of Chinese Medicine. Six-week-old BALB/c mice were purchased from the Guangdong Center for Experimental Animals. All BALB/c mice were kept in a constant temperature at 22 °C and 60% relative humidity with a period of 12 h light–dark cycle and given free access to standard diet and water. Briefly, the cultures of MRSA ATCC 43300 grown overnight at 37 °C in BHI broth were diluted 1:100 using fresh TSB medium and incubated in an incubator shaker for 3 h. Log phase cells were pelleted and washed twice with sterile phosphate buffered saline (PBS) before being resuspended in 100 mL of PBS for further use. Using a Hamilton syringe, BALB/c mice (N = 10) were treated via the lateral tail-vein injection with MRSA ATCC 43300 suspended in PBS at a dose of 109 CFU. A solution of compound 5a in the formulation of 5% Cremophor EL, 5% ethanol and 90% saline was freshly prepared at a concentration of 2 mg/mL on the day of use and used for animal study within 0.5 h. Various treatments including vehicle consisting of 5% CremophorEL, 5% ethanol and 90% saline, compound 5a alone at a concentration of 0.5 mg/kg were administered intraperitoneal injections (IP) per 12 h post infection respectively. The Survival rate of BALB/c mice was recorded at 12 h interval for 4 days after MRSA ATCC 43300 challenge. BALB/c mice were considered dead when they were immobile and no longer responding to physical stimuli. Experimental survival animals will be killed by cervical dislocation to ensure immediate death and not cause unnecessary/prolonged pain to them in accordance with ARRIVE guidelines. Data were analyzed for statistical significance using a log-rank and χ square test with 1 degree of freedom.

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. Fleming A. On the antibacterial action of cultures of a penicillium, with special reference to their use in the isolation of B. influenzae. Rev Infect Dis. 1980;2(1):129–39.

    Article  CAS  PubMed  Google Scholar 

  2. Drawz SM, Bonomo RA. Three decades of beta-lactamase inhibitors. Clin Microbiol Rev. 2010;23(1):160–201.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Koch AL. Bacterial wall as target for attack: past, present, and future research. Clin Microbiol Rev. 2003;16(4):673–87.

    Article  PubMed  PubMed Central  Google Scholar 

  4. Lima LM, Silva B, Barbosa G, Barreiro EJ. β-lactam antibiotics: an overview from a medicinal chemistry perspective. Eur J Med Chem. 2020;208: 112829.

    Article  CAS  PubMed  Google Scholar 

  5. Lim CJ, Kong DC, Stuart RL. Reducing inappropriate antibiotic prescribing in the residential care setting: current perspectives. Clin Interv Aging. 2014;9:165–77.

    PubMed  PubMed Central  Google Scholar 

  6. Dellit TH, Owens RC, McGowan JE, Gerding DN, Weinstein RA, Burke JP, Huskins WC, Paterson DL, Fishman NO, Carpenter CF, Brennan PJ, Billeter M, Hooton TM. Infectious diseases society of america and the society for healthcare epidemiology of America guidelines for developing an institutional program to enhance antimicrobial stewardship. Clin Infect Dis. 2007;44(2):159–77.

    Article  PubMed  Google Scholar 

  7. Jin WB, Xu C, Cheng Q, Qi XL, Gao W, Zheng Z, Chan EWC, Leung Y-C, Chan TH, Wong K-Y, Chen S, Chan K-F. Investigation of synergistic antimicrobial effects of the drug combinations of meropenem and 1,2-benzisoselenazol-3(2H)-one derivatives on carbapenem-resistant Enterobacteriaceae producing NDM-1. Eur J Med Chem. 2018;155:285–302.

    Article  CAS  PubMed  Google Scholar 

  8. Hinchliffe P, González MM, Mojica MF, González JM, Castillo V, Saiz C, Kosmopoulou M, Tooke CL, Llarrull LI, Mahler G, Bonomo RA, Vila AJ, Spencer J. Cross-class metallo-β-lactamase inhibition by bisthiazolidines reveals multiple binding modes. Proc Natl Acad Sci. 2016;113(26):E3745–54.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Bush K. Proliferation and significance of clinically relevant β-lactamases. Ann N Y Acad Sci. 2013;1277:84–90.

    Article  CAS  PubMed  Google Scholar 

  10. Boyd SE, Livermore DM, Hooper DC, Hope WW. Metallo-β-Lactamases: structure, function, epidemiology, treatment options, and the development pipeline. Antimicro Agents Chemotherapy. 2020;64(10):10–128.

    Article  Google Scholar 

  11. Hofer U. Novel metallo-β-lactamase inhibitors. Nat Rev Microbiol. 2022;20(3):125–125.

    PubMed  Google Scholar 

  12. Yue K, Xu C, Wang Z, Liu W, Liu C, Xu X, Xing Y, Chen S, Li X, Wan S. 1,2-Isoselenazol-3(2H)-one derivatives as NDM-1 inhibitors displaying synergistic antimicrobial effects with meropenem on NDM-1 producing clinical isolates. Bioorganic Chem. 2022;129: 106153.

    Article  CAS  Google Scholar 

  13. De Oliveira DMP, Forde BM, Kidd TJ, Harris PNA, Schembri MA, Beatson SA, Paterson DL, Walker MJ. Antimicrobial resistance in ESKAPE pathogens. Clin Microbio Rev. 2020;33(3):10–128.

    Google Scholar 

  14. Linciano P, Cendron L, Gianquinto E, Spyrakis F, Tondi D. Ten years with New Delhi Metallo-β-lactamase-1 (NDM-1): from structural insights to inhibitor design. ACS Infect Dis. 2019;5(1):9–34.

    Article  CAS  PubMed  Google Scholar 

  15. Bush K, Bradford PA. Interplay between β-lactamases and new β-lactamase inhibitors. Nat Rev Microbiol. 2019;17(5):295–306.

    Article  CAS  PubMed  Google Scholar 

  16. Liu B, Trout REL, Chu G-H, McGarry D, Jackson RW, Hamrick JC, Daigle DM, Cusick SM, Pozzi C, De Luca F, Benvenuti M, Mangani S, Docquier J-D, Weiss WJ, Pevear DC, Xerri L, Burns CJ. Discovery of Taniborbactam (VNRX-5133): a broad-spectrum serine- and Metallo-β-lactamase inhibitor for carbapenem-resistant bacterial infections. J Med Chem. 2020;63(6):2789–801.

    Article  CAS  PubMed  Google Scholar 

  17. Hecker SJ, Reddy KR, Lomovskaya O, Griffith DC, Rubio-Aparicio D, Nelson K, Tsivkovski R, Sun D, Sabet M, Tarazi Z, Parkinson J, Totrov M, Boyer SH, Glinka TW, Pemberton OA, Chen Y, Dudley MN. Discovery of cyclic boronic acid QPX7728, an ultrabroad-spectrum inhibitor of serine and metallo-β-lactamases. J Med Chem. 2020;63(14):7491–507.

    Article  CAS  PubMed  Google Scholar 

  18. Li X, Zhao J, Zhang B, Duan X, Jiao J, Wu W, Zhou Y, Wang H. Drug development concerning metallo-β-lactamases in gram-negative bacteria. Front Microbiol. 2022;13:959107.

    Article  PubMed  PubMed Central  Google Scholar 

  19. Jin WB, Xu C, Cheung Q, Gao W, Zeng P, Liu J, Chan EWC, Leung Y-C, Chan TH, Wong K-Y, Chen S, Chan K-F. Bioisosteric investigation of ebselen: Synthesis and in vitro characterization of 1,2-benzisothiazol-3(2H)-one derivatives as potent New Delhi metallo-β-lactamase inhibitors. Bioorg Chem. 2020;100: 103873.

    Article  CAS  PubMed  Google Scholar 

  20. Jin WB, Xu C, Qi XL, Zeng P, Gao W, Lai KH, Chiou J, Chan EWC, Leung Y-C, Chan TH, Wong K-Y, Chen S, Chan K-F. Synthesis of 1,3,4-trisubstituted pyrrolidines as meropenem adjuvants targeting New Delhi metallo-β-lactamase. New J Chem. 2021;45(7):3515–34.

    Article  CAS  Google Scholar 

  21. Adibpour N, Khalaj A, Rajabalian S. Synthesis and antibacterial activity of isothiazolyl oxazolidinones and analogous 3(2H)-isothiazolones. Eur J Med Chem. 2010;45(1):19–24.

    Article  CAS  PubMed  Google Scholar 

  22. Luna BL, Garcia JA, Huang M, Ewing PJ, Valentine SC, Chu Y-M, Ye Q-Z, Xu HH. Identification and characterization of novel isothiazolones with potent bactericidal activity against multi-drug resistant Acinetobacter baumannii clinical isolates. Int J Antimicrob Agents. 2019;53(4):474–82.

    Article  CAS  PubMed  Google Scholar 

  23. Verderosa AD, Hawas S, Harris J, Totsika M, Fairfull-Smith KE. Isothiazolone-Nitroxide hybrids with activity against antibiotic-resistant Staphylococcus aureus Biofilms. ACS Omega. 2022;7(6):5300–10.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  24. Jin WB, Wang Z, Yang W, Zhang D, Ning JH, Ke J, Hou AG, Chen LY, Ma YS. Selective [3 + 2] cycloaddition reaction of isothiazol-3(2h)-ones with in situ formed azomethine ylide to thiazolidines and oxazolidines. J Heterocycl Chem. 2023;60(8):1383–93.

    Article  CAS  Google Scholar 

  25. Tyers M, Wright GD. Drug combinations: a strategy to extend the life of antibiotics in the 21st century. Nat Rev Microbiol. 2019;17(3):141–55.

    Article  CAS  PubMed  Google Scholar 

  26. Zhang J, Zhao X, Cappiello JR, Yang Y, Cheng Y, Liu G, Fang W, Luo Y, Zhang Y, Dong J, Zhang L, Sharpless KB. Identification of simple arylfluorosulfates as potent agents against resistant bacteria. Proceed Nat Acad Sci USA. 2021;118(28):e2103513118.

    Article  CAS  Google Scholar 

  27. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharma Analy. 2016;6(2):71–9.

    Article  Google Scholar 

  28. Yuan Z, Wang J, Qu Q, Zhu Z, Xu M, Zhao M, Sun C, Peng H, Huang X, Dong Y, Dong C, Zheng Y, Yuan S, Li Y. Celastrol Combats Methicillin-Resistant Staphylococcus aureus by Targeting Δ1-Pyrroline-5-carboxylate dehydrogenase. Adv Sci. 2023;10(25):2302459.

    Article  CAS  Google Scholar 

  29. Yang W, Wang W, Cai S, Li P, Zhang D, Ning J, Ke J, Hou A, Chen L, Ma Y, Jin W. Synthesis and in vivo antiarrhythmic activity evaluation of novel scutellarein analogues as voltage-gated Nav15 and Cav12 channels blockers. Molecules. 2023;28(21):7417.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Chan K-F, Sun N, Yan S-C, Wong ILK, Lui H-K, Cheung K-C, Yuan J, Chan F-Y, Zheng Z, Chan EWC, Chen S, Leung Y-C, Chan TH, Wong K-Y. Efficient synthesis of amine-linked 2,4,6-Trisubstituted pyrimidines as a new class of bacterial FtsZ inhibitors. ACS Omega. 2017;2(10):7281–92.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Lui HK, Gao W, Cheung KC, Jin WB, Sun N, Kan JWY, Wong ILK, Chiou J, Lin D, Chan EWC, Leung YC, Chan TH, Chen S, Chan KF, Wong KY. Boosting the efficacy of anti-MRSA β-lactam antibiotics via an easily accessible, non-cytotoxic and orally bioavailable FtsZ inhibitor. Eur J Med Chem. 2019;163:95–115.

    Article  CAS  PubMed  Google Scholar 

  32. Xu C, Chen K, Chan KF, Chan EWC, Guo X, Chow HY, Zhao G, Zeng P, Wang M, Zhu Y, Li X, Wong K-Y, Chen S. Imidazole type antifungal drugs are effective colistin adjuvants that resensitize colistin-resistant enterobacteriaceae. Advanced Therapeutics. 2020;3(9):2000084.

    Article  CAS  Google Scholar 

Download references

Funding

We acknowledge the support from the National Natural Science Foundation of China (Grant No. 82104067), Bioactive Ethnopharmacol Molecules Chemical Conversion and Application Innovation Team of Department of Education of Yunnan Province, Yunnan Provincial Key Laboratory of Molecular Biology for Sinomedicine (2019DG016), Yunnan Provincial Joint Project of Traditional Chinese Medicine (202001AZ070001-091) and Natural Science Foundation of Yunnan (202001AU070133).

Author information

Author notes

  1. Wenbin Jin and Chen Xu contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of External Drug Delivery System and Preparation Technology in Universities of Yunnan and Faculty of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, Yunnan, China

    Wenbin Jin, Die Zhang, Jinhua Ning, Jin Ke, Anguo Hou & Linyun Chen

  2. State Key Laboratory of Chemical Biology and Drug Discovery and Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China

    Wenbin Jin, Chen Xu, Ning Dong, Kaichao Chen & Kin-Fai Chan

  3. School of Medicine, Jiangsu University, Zhenjiang, Jiangsu, China

    Chen Xu

  4. Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, China

    Sheng Chen

  5. Department of Medical Microbiology, School of Biology and Basic Medical Sciences, Suzhou Medical College of Soochow University, Suzhou, China

    Ning Dong, Yunbing Li & Guangfen Zhang

Authors
  1. Wenbin Jin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Chen Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ning Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kaichao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Die Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jinhua Ning
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yunbing Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Guangfen Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Jin Ke
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Anguo Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Linyun Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Sheng Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Kin-Fai Chan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

WBJ, DZ, JHN, and JK synthesized, purified the compounds and carried out 1H-NMR and 13C-NMR. KCC, AGH, and LYC performed animal experiments. CX, ND, YBL, GFZ performed the antimicrobial susceptibility testing experiments. WBJ and CX wrote and edited the manuscript. KFC and SC designed the experiments and supervised the whole project. All authors reviewed the manuscript.

Corresponding authors

Correspondence to Wenbin Jin, Sheng Chen or Kin-Fai Chan.

Ethics declarations

Ethics approval and consent to participate

All studies on animals were performed in compliance with Guide for the Care and Use of Laboratory Animals: Eighth Edition (2011) for the care and use of laboratory animals. Animal survival studies were approved by the Animal Research Ethics Committee of Yunnan University of Chinese Medicine (Approval ID: R-062020G087). The study is also in accordance with ARRIVE guidelines and the revised Animals (Scientific Procedures) Act 1986 in the UK and Directive 2010/63/EU in Europe.

Consent for publication

Not applicable.

Competing interests

All authors disclosed no relevant relationships.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Supplementary Information

Additional file 1: Figure S1.

1H and 13C NMR spectra of 2-(4-chlorophenyl)isothiazol-3(2H)-one (4a). Figure S2. 1H and 13C NMR spectra of 2-((4-methoxyphenyl)sulfonyl)isothiazol-3(2H)-one (4b). Figure S3. 1H and 13C NMR spectra of 2-(4-bromobenzyl)isothiazol-3(2H)-one (4c). Figure S4. 1H and 13C NMR spectra of 5-chloro-2-(4-chlorophenyl)isothiazol-3(2H)-one (5a). 13C NMR (101 MHz, CHLOROFORM-d) 167.5, 140.2, 135.1, 133.0, 129.5, 125.9, 114.7. Figure S5. 1H and 13C NMR spectra of 5-chloro-2-((4-methoxyphenyl)sulfonyl)isothiazol-3(2H)-one (5b). Figure S6. 1H and 13C NMR spectra of 5-chloro-2-(3-chloro-4-methoxyphenyl)isothiazol-3(2H)-one (5c). Figure S7. 1H and 13C NMR spectra of 5-chloro-2-cyclohexylisothiazol-3(2H)-one (5d). Figure S8. 1H and 13C NMR spectra of 5-chloro-2-(quinolin-8-ylsulfonyl)isothiazol-3(2H)-one (5e). Figure S9. 1H and 13C NMR spectra of 5-chloro-2-octylisothiazol-3(2H)-one (5f). Figure S10. 1H and 13C NMR spectra of 5-chloro-2-(prop-2-yn-1-yl)isothiazol-3(2H)-one (5g). Figure S11. 1H and 13C NMR spectra of 4,5-dichloro-2-(4-chlorophenyl)isothiazol-3(2H)-one (6). Figure S12. 1H and 13C NMR spectra of 4-chloro-2-(4-chlorophenyl)isothiazol-3(2H)-one (7). Figure S13. 1H and 13C NMR spectra of 4-bromo-2-(4-chlorophenyl)isothiazol-3(2H)-one (8). Figure S14. 1H and.13C NMR spectra of 5-chloro-2-(4-chlorophenyl)isothiazol-3(2H)-one 1-oxide (9).

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Jin, W., Xu, C., Dong, N. et al. Identification of isothiazolones analogues as potent bactericidal agents against antibiotic resistant CRE and MRSA strains. BMC Chemistry 17, 183 (2023). https://0-doi-org.brum.beds.ac.uk/10.1186/s13065-023-01100-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://0-doi-org.brum.beds.ac.uk/10.1186/s13065-023-01100-3

Keywords

BMC Chemistry

ISSN: 2661-801X

Contact us